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KMID : 0363620090300050061
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Journal of Korean Oriental Medicine
2009 Volume.30 No. 5 p.61 ~ p.76
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Effects of Scutellaria Radix Extract on the Membranous Nephropathy Induced by Cationic Bovine Serum Albumin in Mouse Model
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Kim Man-Ho
Cho Chung-Sik
Kim Chul-Jung
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Abstract
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Background: Membranous nephropathy (MN) is the most common cause of adult nephrotic syndrome worldwide and has been defined as granular subepithelial deposition of immune complexes along the glomerular basement membrane (GBM). MN has few known treatments and gives rise to side effects under treatment with steroids and immunosuppressives.
Objective: The purpose of this experimental study was to demonstrate the effects of Scutellariae Radix extract (SRE) treatment on MN mouse model induced by cBSA. Methods: We divided mice into 4 groups. The Normal group had no treatment. We induced MN mouse model to the other 3 groups by injecting cBSA into the abdominal cavity. The control group was treated with cBSA (10 mg/kg, i.p.) only. The second group, ’SRE-250’, was treated with cBSA (10 mg/kg, i.p.) and SRE (250 mg/kg, p.o.). The third group, ’SRE-500’, was treated with cBSA (10 mg/kg, i.p.) and SRE (500 mg/kg, p.o.). After cBSA and SRE treatment for 4 weeks, gain in body weight, 24hrs proteinuria, serum albumin, total cholesterol, triglyceride, BUN and creatinine of all groups were measured. TNF-, IL-6, IL-1, IL-10, IFN-, IgA, IgM and IgG levels of all groups were gauged. H&E staining and electron microscopy of the kidney were observed.
Results: SRE showed significant decrease in the 24hrs proteinuria, serum triglyceride, BUN, TNF-, IL-6, serum IgA, IgM and IgG levels compared with the control group. SRE showed increase in the serum IL-10 and IFN- levels compared with control on RT-PCR. SRE considerably decreased in the thickening of the GBM on H&E staining and deposition of electron-density on electron microscopy of the kidney compared with the control.
Conclusions: According to the above results, it is suggested that SRE decreases the symptoms of MN induced by cBSA in mouse model. Therefore, SRE seems to be applicable to MN in clinical practice.
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KEYWORD
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Scutellariae Radix extract (SRE), membranous nephropathy (MN), cationic bovine serum albumin (cBSA)
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